THE STUDY OF HLA-B*44~C HAPLOTYPE POLYMORPHISM IN THE CROATIAN POPULATION
The aim of the study was to investigate the distribution of HLA-B*44 alleles and determine the common HLA-B*44~C haplotypes in the Croatian population.
The subjects included in this study (N=316) were randomly chosen B*44-positive healthy individuals previously typed for the HLA-A, -B, -C and -DRB1 loci. The high resolution level typing of HLA-B and -C loci was performed using the Polymerase Chain Reaction – Sequence Specific Primers (PCR-SSP) method.
Among five detected HLA-B*44 alleles the most frequent one was HLA-B*44:02 (41.40%) followed by -B*44:03 (25.70%) and -B*44:27 (18.50%). The HLA-B*44:06 allele was detected only once (0.30%). The analysis of the HLA-C alleles’ distribution showed a predominance of a single HLA-C allele in all four subgroups: HLA-C*05:01 for the HLA-B*44:02-positive individuals, HLA-C*04:01 among HLA-B*44:03-positive subjects, HLA-C*02:02 in the HLA-B*44:05-positive group and HLA-C*07:04 among individuals carrying HLA-B*44:27.
The potential application of these data can be found in pre-transplant management of HLA-B*44-positive patients in both hematopoietic stem cell and solid organ transplantation program.