INHIBITION OF PARP ACTIVITY DOES NOT AFFECT THE DIFFERENTIATION PROCESSES CAUSED BY RETINOIC ACID IN SH-SY5Y CELLS
Differentiation is a complex process by which cells become specialized in their physiological functions. As this process is followed by a decrease in proliferation ability, induction of differentiation could be an ideal cancer treatment. We analysed the effects of a common differentiation agent, retinoic acid on neuroblastoma SH-SY5Y cells under the conditions of poly(ADP-ribose) polymerase (PARP) inhibition. Namely, PARP1 can be indirectly involved in processes of chromatin remodelling. Cells differentiated after retinoic acid treatment into neural cells, with neurite outgrowth, proliferation arrest and induction of tissue plasminogen activator. PARP inhibition did not influence the process of differentiation. Analysis of gene expression revealed the involvement of TGF β, Notch and pathways related to epithelial-mesenchymal transition to be involved in RA-dependent differentiation.